Search results for "CITRUS CLEMENTINA"

Untargeted metabolomics of rind essential oils allowed to differentiate two closely related clementine varieties

2021

[EN] Chemical characterization of clementine varieties (Citrus clementina Hort. ex Tan.) essential oils (EO) can lead to variety identification and valorization of their potential use in food and aroma industries. The goal of this study was the chemometric discrimination between two very closely related and morphologically identical clementine varieties, Clemenules (NL) and Clemenpons (PO), based on their rind EO, to identify the differential volatile organic compounds (VOCs) and to determine their antioxidant capacity. EO rind volatile profile was determined by gas chromatography coupled to mass spectrometry in Citrus fruit at different ripening stages grown two independent years in two di…

Monitoring and cellular characterisation of male gametic embryogenesis in Citrus clementina.

2004

The effect of light quality on anther culture of Citrus clementina Hort. ex Tan.

2005

This pre lim i nary re search re ports re sults on the in flu ence of light qual ity on an ther cul ture of Cit rus clementina Hort. ex Tan., cultivar Nules. Af ter one month of cul ti va tion in dark - ness, four light quali ties were tested: White, Red, Far-Red and Blue. Con tin u ous Dark ness and White light un der photoperiod of 16 hrs were used as a con trol con di tions. Ga metic embryoids and embryogenic cal lus were ob tained only un der photoperiodic con di tions of White light, sug gest ing that the al - ter na tion of light and dark can be used for the pro cess of ga - metic embryogenesis in Cit rus.

Anther culture in Citrus clementina: a way to regenerate tri-haploids

2005

Abstract. Regenerants from anther culture of Citrus clementina Hort. ex Tan. cvv. Nules, SRA 63, and Monreal were obtained in different experiments from 1994 to 2002. Genetic analysis of 37 such regenerants was carried out using 4 microsatellite markers that were heterozygous in the parental genotypes. The results showed that in all cases but one the regenerants carried only one or the other allele of the parental genotype, and were therefore homozygous and produced through a process of gametophytic embryogenesis. Ploidy analysis by flow cytometry of 94 regenerants showed that as many as 82% of them were tri-haploids, rather than haploids or doubled-haploids as expected, with other ploidy …

CHARACTERIZATION OF LEAF ESSENTIAL OIL COMPOSITION OF HOMOZYGOUS AND HETEROZYGOUS Citrus clementina HORT. EX TAN. AND OF ITS ANCESTORS

2009

Effects of high and low intensità magnetic fields on pollen behaviour of Citrus clementina Hort. ex Tan. and Pinus pinea L.

2003

Primeras etapas de la embriogenesis de microsporas en coltivo in vitro de anteras de Citrus clementina Hort. ex Tan.

2003

The oil composition of homozygous and heterozygous plants of Citrus clementina Hort. ex Tan.".

2008

Direct Organogenesis from Cotyledons in Cultivars of Citrus clementina Hort. ex Tan

2011

An efficient protocol to induce shoot buds regeneration in Citrus clementina cultivars (“Monreal”, “SRA 63” and “SRA 64”) by direct organogenesis has been developed using cotyledons as explants. Cotyledons transversely cut in three segments and entire ones were cultured on Murashige and Skoog (1962) solidified medium containing vitamins, 500 mg·l−1 malt extract, 50 g·l−1 sucrose and supplemented with three different concentrations of BAP (8.8, 13.2 and 17.6 μM). In all three cultivars the entire cotyledons showed more shoot morphogenic potential than transversely cut ones and after 60 incubation days the optimum BAP concentration was 17.6 μM in “Monreal” (50% ± 2.89% of frequency regenerati…

Characterization of Leaf Essential Oil Composition of Homozygous and HeterozygousCitrus clementinaHort. Extan. and its Ancestors

2013

Clementine is a natural tangor, resulting from an interspecific cross between mandarin and sweet orange. Gametic embryogenesis, allowing the single-step development of complete homozygous line from the heterozygous parents, increases the efficiency of perennial crop breeding programs. Tri-haploids have been regenerated through pollen embryogenesis (specifically, by anther culture) of Citrus clementina Hort. ex Tan., cv. Nules. Two of them (HOMO1 and HOMO2) have been acclimatized and grafted in vivo in 2000. Research regarding the chemical characteristics of plant regenerated by pollen embryogenesis or gynogenesis are useful to study the “gametoclonal variation”, as well as for further appli…